An Attempt for in Situ Bio-Root Regeneration by Using Treated Dentin Matrix and Dental Pulp Stem Cells in Albino Rats

Amani Mohamad, Senior Teaching Assistance of Oral Biology, Mansoura University, Faculty of Dentistry.Follow
Ahmad A. Emam, Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura university
Eman Hany, Assistant Professor of oral Biology, Mansoura University, Faculty of Dentistry.
Mohamed Abdulrahman, Professor of oral Biology, Mansoura University, Faculty of Dentistry

Article Type

Original Article

Section/Category

Oral Biology

Abstract

Objectives: This study was conducted to prepare and characterize root-shaped treated dentin matrix (TDM) scaffolds, and to evaluate their efficacy together with seeded dental pulp stem cells (DPSCs) as potential bio-roots in extraction sockets of rat models.

Materials and Methods: Root-shaped TDM specimens were prepared and characterized. Following extraction of maxillary incisors, the alveolar sockets were implanted with TDM segments measuring 8 mm in length, either alone (TDM group) or seeded with dental pulp stem cells (TDM/DPSCs group). Animals were euthanized at two and six weeks post-implantation, specimens were processed for histological, histochemical and immunohistochemical analyses.

Results: TDM group at 2 weeks showed poor regeneration on the inner and outer surfaces of the implanted scaffolds, while at 6 weeks early connective tissue formation and collagen fiber deposition were observed. TDM/DPSCs group at 2 weeks demonstrated predentin-like tissue formation and preliminary PDL-like tissues, and by 6 weeks exhibited pronounced regenerative outcomes, including dentin-like tissue formation, odontoblast-like cells lining the TDM surface, organized pulp-like tissue, and well-arranged PDL-like collagen fibers at outer surface. Statistical analysis of Masson’s trichrome and Dentin sialophosphoprotienn immunohistochemical staining revealed a statistically significant difference between both groups, with the TDM/DPSCs group showing superior collagen organization and higher odontogenic marker expression in comparison with the TDM group.

Conclusion: The TDM/DPSCs construct effectively promotes dentin and periodontal tissue regeneration in rat incisor extraction sockets. This strategy represents a feasible and promising tissue engineering approach for tooth root regeneration and may have potential clinical applications in the treatment of tooth loss.

Keywords

Extraction socket, Bioactive scaffolds, Extra-cellular matrix, Dental mesenchymal stem cells, Dentin sialophosphoprotien

How to Cite This Article

Mohamad A , Emam A , Hany E , Abdulrahman M . An Attempt for in Situ Bio-Root Regeneration by Using Treated Dentin Matrix and Dental Pulp Stem Cells in Albino Rats. Mans J Dent. 2026; 13(2):
Available at: https://doi.org/10.61793/2812-5479.1187