Effect of Biodentine and Treated dentine matrix on viability and proliferation of human dental pulp stem cells

Nada Barakat, Demonstrator, Oral Biology Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. El Mansoura 1, Dakahlia Governorate, postal code: 35516Follow
Rana El-Qashty, Assistant Professor, Oral Biology Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Mohamad E. Helal, Professor, Oral Biology Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.

Article Type

Original Article

Section/Category

Oral Biology

Abstract

Objective: The present study aimed to assess the dose-dependent effect of Biodentine (BD) and human-treated dentin matrix (hTDM) on the proliferation of human dental pulp stem cells (hDPSCs).

Materials and methods: hTDM was prepared and characterized. The concentration of proteins released from BD and hTDM extracts were quantified using the bicinchoninic acid (BCA) assay after 3 and 7 days. hDPSCs were successfully isolated and characterized, then treated with various concentrations of BD extracts (20, 2, and 0.2 mg/mL) and hTDM extracts (100%, 80%, 60%, 40%, 20%, and 10%). Cell viability was determined after 7 days of incubation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and represented as a percentage relative to untreated control groups. One-way ANOVA statistical analysis followed by post-hoc Tukey test were performed.

Results: High-concentration BD (20 mg/mL) significantly reduced hDPSCs viability, indicating cytotoxicity. In contrast, lower BD concentrations (2 and 0.2 mg/mL) slightly enhanced cell proliferation compared with the negative control, with the 0.2 mg/mL exhibiting the most significant enhancement. For hTDM, concentrated extracts (100–40%) significantly decreased cell viability. However, diluted extracts (20% and 10%) preserved high proliferation rates, with no significant differences between them.

Conclusions:

This study demonstrated the favorable invitro biocompatibility of BD, a commercially available tricalcium silicate cement, and hTDM, an innovative natural biomaterial, on hDPSCs. Lower concentrations, particularly 0.2 mg/mL BD and 10% hTDM, exhibited superior biological outcomes compared with higher doses. Appropriate concentration optimization is essential to maximize regenerative benefits and enable successful clinical translation.

Keywords

Regenerative endodontic, MTT, pulp capping, biocompatibility, dentin bridge

How to Cite This Article

Barakat N , El-Qashty R , Helal M . Effect of Biodentine and Treated dentine matrix on viability and proliferation of human dental pulp stem cells. Mans J Dent. 2026; 13(2):
Available at: https://doi.org/10.61793/2812-5479.1185