Effect of adjunctive incorporation of nanoparticles of amorphous calcium phosphate and chlorhexidine on properties of an etch-and-rinse bonding agent

Hussein Elshikha, Postgraduate student, Department of Dental Biomaterials, Faculty of Dentistry, Mansoura University, Mansoura, Dakahlia, EgyptFollow
Elsayed Gad, Professor, Department of Dental Biomaterials, Faculty of Dentistry, Mansoura University, Mansoura, Dakahlia, Egypt
Noha Sheta, Lecturer, Department of Dental Biomaterials, Faculty of Dentistry, Mansoura University, Mansoura, Dakahlia, Egypt

Article Type

Original Article

Section/Category

Dental Biomaterials

Abstract

Objective: To assess the effect of the addition of nanoparticles of amorphous calcium phosphate (NACP) and chlorhexidine (CHX) on the properties of an etch-and-rinse adhesive regarding the bond strength to caries-affected dentine (CAD) and the NACP remineralization efficacy.

Materials and Methods: Dentine surfaces from 80 human molars were subjected to pH cycling and then divided into 4 groups (n=20): adhesive without additives (control group), 5% wt/wt NACP added to the adhesive (NACP group), 0.1% CHX added to the adhesive (CHX group), and addition of 5%wt/wt NACP and 0.1% CHX to the adhesive (NACP+CHX group). After acid etching, the adhesive for each group was applied and composite resin was built up respectively. Following a storage period of 6 months, microtensile bond strength (µTBS) and scanning electron microscope (SEM) with energy dispersive X-ray (EDX) were done. The data were analyzed using One-way ANOVA, Monte Carlo tests and the significance level was set at ≤0.05.

Results: The µTBS results showed the highest mean among CHX group (30.3 ±5.03 MPa) followed by NACP+CHX group (25.11 ±13.79 MPa) then NACP group (16.28 ±2.65 MPa) and the control group (14.72 ±3.05 MPa). SEM with EDX demonstrated the highest mean for Calcium (Ca) and Phosphorous (P) levels among NACP group (43.04 ±0.021wt%) (14.76 ±0.25wt%) followed by control group (32.50 ±0.014wt%) (13.18 ±0.07wt%) then CHX group (31.32 ±0.02wt%) (13.15 ±0.035wt%) and NACP+CHX group (31.30 ±0.14wt%) (15.35 ±0.06wt%).

Conclusions: The addition of chlorhexidine and nanoparticles of amorphous calcium phosphate to the adhesive maintained the bond strength to caries-affected dentine by significantly enhancing its efficacy (P

Keywords

Antimicrobial activity, Caries-affected dentine, Dental adhesion, Remineralization.

How to Cite This Article

Elshikha H , Gad E , Sheta N . Effect of adjunctive incorporation of nanoparticles of amorphous calcium phosphate and chlorhexidine on properties of an etch-and-rinse bonding agent. Mans J Dent. 2024; 11(3):
Available at: https://doi.org/10.61793/2812-5479.1131