Biocompatibility Evaluation of EDTA and Chitosan Nanoparticles Gel Forms: In vivo histopathological study

Alaa H. Youssef, Demonstrator, Demonstrator of Endodontics, Faculty of Oral and Dental Medicine, Zagazig University, Zagazig, EgyptFollow
Doaa A. Labah, Professor, Department of Oral Biology, Faculty of Oral and Dental Medicine, Zagazig University, Zagazig, Egypt
Abeer A. Elgendy, Professor, Department of Endodontics, Faculty of Oral and Dental Medicine, Zagazig University, Zagazig, Egypt
Amany E. Badr, Professor, Department of Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt

Article Type

Original Article

Section/Category

Endodontics

Abstract

Objectives: The purpose of this study was to evaluate and compare biocompatibility of gel form chelating agents (EDTA and chitosan NPS) after implantation of polyethylene tubes in rats. Materials and methods: Twenty albino rats were included in this study. Three horizontal incisions were performed on dorsal surface of each rat to receive three polyethylene tubes as subcutaneous implants; two of them filled with tested materials and one empty tube served as control. After 2 and 4 weeks of subcutaneous implantation, 10 rats were sacrificed and implanted polyethylene tubes with surrounding tissues were dissected with 1 cm safety margin. The specimens were submitted to histological preparation and stained with hematoxylin and eosin then investigated using light microscope. The number of inflammatory cells were assessed and statistically compared by One-way analysis of variance (ANOVA), followed by the post hoc Tukey’s test (P.05). After 4 weeks of implantation, the severity of inflammatory cell infiltration was decreased in all groups. Conclusions: All tested materials produced inflammatory reaction. The tissue inflammatory reaction induced by tested materials showed a gradual reduction with time. 0.2% chitosan NPS gel demonstrated superior biocompatibility compared to EDTA gel.

Keywords

EDTA gel, chitosan NPS gel, biocompatibility, subcutaneous implantation, rats.

How to Cite This Article

Youssef A , Labah D , Elgendy A , Badr A . Biocompatibility Evaluation of EDTA and Chitosan Nanoparticles Gel Forms: In vivo histopathological study. Mans J Dent. 2024; 11(3):
Available at: https://doi.org/10.61793/2812-5479.1130