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Article Type

Original Article

Section/Category

Oral Biology

Abstract

Objective: This in vitro investigation's goal was to ascertain how well dental pulp stem cell exosomes (DPSC-exo) modulate endogenous dental pulp stem cells (DPSCs) and direct their navigation toward mineralized tissue regeneration, either alone or in conjunction with mineral trioxide aggregate (MTA). Materials and methods: For this investigation, DPSCs were harvested using rat incisors. To extract exosomes from DPSCs, an ultracentrifugation method was employed. Flow cytometry was used for the characterization of DPSCs-exo, and transmission electron microscopy (TEM) was used to measure their size. DPSCs were incubated in standard culture media and split up into three groups. The first one served as the negative control group and contained only DPSCs; in the second group, DPSCs were treated with exosomes; and in the third group, DPSCs were treated with both MTA and exosomes. Then, the cell viability assay was used to assist cell proliferation. Osteogenic differentiation has been assisted by quantitative reverse transcription chain reaction (qRT-PCR). The overall significance between groups was examined using a one-way ANOVA test, followed by a post hoc test (P≤.05). Results: The pulp stem cell exosomes trigger the proliferation of DPSCs. The presence of MTA in the combination with exosomes results in a higher proliferation rate (P

Keywords

Exosomes; Dental; Stem cells; MTA; regenerative endodontics

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