Immunohistochemical Assessment of Angiogenesis and Cell Proliferation in Aggressive versus Non-aggressive Central Giant Cell Granuloma.

Mena F. Mesiha, Postgraduate MSc student, Department of Oral Pathology Dentistry, Faculty of Dentistry, Mansoura University 35516, Mansoura, EgyptFollow
Mahmoud F. El Sherbeny, Lecturer, Department of Oral Pathology, Faculty of Dentistry, Mansoura University 35516, Mansoura, Egypt.
Sherif Y. El Nagdy, Professor, Department of Oral Pathology Dentistry, Faculty of Dentistry, Mansoura University 35516, Mansoura, Egypt.

Article Type

Original Article

Section/Category

Oral Pathology

Abstract

Objective: The current study investigated the expression of MCM3 and CD31 in aggressive versus non-aggressive central giant cell granuloma (CGCG ) Materials and methods: Thirty-three paraffin blocks for CGCG cases were collected from Oral Pathology Departments, Faculty of Dentistry, Mansoura, and Alexandria Universities. The Immunohistochemical staining for CD31 and MCM 3 was performed, assessed, and scored. Results: Mean age of the studied cases was 35.6±9.1 years. The lesions that occurred in the mandible represented the majority of cases [27 cases (81.8%)]. The study revealed an association between aggressiveness and the presence of large swelling, pain, cortical perforation, root resorption, and recurrence where they were significantly higher in group 1 (aggressive cases) than in group 2 (non-aggressive cases). Group 1 revealed histologically numerous MNGCs, more cellular connective tissue Stroma, numerous vascularity, and areas of Hemorrhage. Our study showed a highly statistically significant association between MCM3 expression and size and recurrence of the lesion. Statistically, a significant correlation existed between aggressive cases and MCM3 expression. All aggressive cases were associated with a high reaction of MCM3. Comparisons of size and recurrence of the lesions with CD31 expression revealed a highly significant association with high marker reaction in large-size lesions and recurrent cases. The Association between CD31 expression and the presence of aggressiveness was significant. All aggressive cases were associated with a high reaction of CD31 and showed a significant increase in MVD. MCM3 showed a highly significant positive correlation with CD31. Conclusions: Highly significant correlation existed between cell proliferation represented by MCM3 expression and angiogenesis represented by CD31 expression in CGCGs.

How to Cite This Article

Mesiha M F, El Sherbeny M F, El Nagdy S Y. Immunohistochemical Assessment of Angiogenesis and Cell Proliferation in Aggressive versus Non-aggressive Central Giant Cell Granuloma.. Mans J Dent. 2022; 9(4): 162-168.
Available at: https://doi.org/10.21608/mjd.2022.150755.1062